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Welcome! Validated Biosystems is an international consulting firm that has been providing expert guidance in downstream processing since 1987. We provide a range of resources to help you develop robust downstream manufacturing procedures quickly and efficiently, including a free downstream processing library on this website. We invite you to use these resources as they serve you best.

Purification Tools for Monoclonal Antibodies.

New Publications on Downstream Processing
Morrison, C. J., Gagnon, P., Cramer, S. M., Unique selectivity windows using selective displacers/eluents and mobile phase modifiers on hydroxyapatite, J.Chromatogr. A, 1217 6484-6495

Gagnon, P., 2010, Dissociation of antibody-contaminant complexes with hydroxyapatite, Bioprocessing J., 9(4) 14-24

Gagnon, P., 2010, Monoliths open the door to key growth sectors, Bioprocess International, 8(10) 20-23

Gagnon, P., 2010, How to choose an industrial cation exchanger for purification of IgG, BioProcess International, 8(9) 22-34

Previous publications are posted in our Downstream Processing Library.

In press
Morrison, C. J., Gagnon, P., Cramer, S. M., 2010, Purification of monomeric mAb from associated aggregates using selective desorption chromatography in hydroxyapatite systems, Biotechnol Bioeng., doi: 10.1002/bit.22971

Gagnon, P., Hensel, F., Lee, S., Zaidi, S., Chromatographic behavior of IgM:DNA complexes, J. Chromatogr. A, doi:10.1016/j.chroma.2010.12.066

Gagnon, P., Rodriquez, G., Zaidi, S., Dissociation and fractionation of heavy and light chains from IgG monoclonal antibodies, J. Chromatogr. A, doi:10.1016/j.chroma.2010.12.008

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Validated Biosystems Resource Guide for Downstream Processing
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Our downstream processing library currently includes articles addressing hydrophobic interaction chromatography, cation exchange chromatography, anion exchange chromatography, chromatofocusing, hydroxyapatite chromatography, fluorapatite chromatography, immobilized metal affinity chromatography, size exclusion chromatography, hydrophilic interaction chromatography, bio-affinity chromatography, protein A affinity chromatography, differential solubility chromatography, endotoxin removal, endotoxin complexation with proteins, DNA removal, DNA complexation with proteins, virus purification, virus removal, virus inactivation, contaminant complexation with proteins, protein formulation, protein stabilization, protein solubility determination, influence of matrix structure on chromatographic performance, degradation of chromatography media, breakdown of chromatography media, validation of chromatography media, process validation, purification process validation, influence of gradient conditions on chromatographic performance, linear versus step gradients, determination of protein binding capacity, membrane filtration losses, protein conjugation, development of immunoconjugates, fluorescent conjugates, immunotoxin conjugation, PEG conjugation, formulation, validation of protein purification processes, validation of virus purification processes, orthogonal process design, orthogonal process development, mass transport in chromatography media, diffusion and convection in chromatography media, monolithic chromatography media, chromatography on monoliths, membrane chromatography media, biotech consultants, biotechnology consultants, process development consultants, purification process development consultants, purification consultants, consultants for development of protein purification methods, consultants for development of virus purification methods, technical consultants for evaluation of venture opportunities, media scouting, courses in downstream processing, antibody purification with hydroxyapatite, aggregate removal with hydroxyapatite, leached protein A removal with hydroxyapatite, virus removal with hydroxyapatite, aggregate removal with fluorapatite, antibody purification with fluorapatite, endotoxin removal with hydroxyapatite